A Novel Marker?
Philip J. Williams; Khalid Gumaa; Marco Scioscia; Christopher W. Redman; Thomas W. Rademacher
From the Department of Molecular Pathology (P.J.W., M.S., T.W.R.), Molecular Medicine Unit, Royal Free and University College Medical School, London, United Kingdom; the College of Medicine and Medical Sciences (K.G.), Arabian Gulf University, Manama, Kingdom of Bahrain; the Department of Obstetrics and Gynaecology (M.S.), University of Bari, Bari, Italy; and the Nuffield Department of Obstetrics and Gynaecology (C.W.R.), University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom.
A state of insulin resistance has been demonstrated in active preeclampsia, and women with clinical evidence of insulin resistance are at higher risk to develop this syndrome during pregnancy. Recently, inositol phosphoglycan P-type, a putative second messenger of insulin action, has been implicated in the pathophysiology of preeclampsia and is increased in the placenta, amniotic fluid, and maternal urine of preeclamptic women compared with normal pregnant women. We report here a case–control study to assess the potential of urinary levels of inositol phosphoglycan P-type as a screening test for preeclampsia. Twenty-seven preeclamptic women and 47 healthy pregnant women were recruited. A polyclonal antibody-based ELISA was developed to detect levels of inositol phosphoglycan P-type in urine. Its content in urinary specimens was found to be 30-fold higher in preeclamptic subjects than control subjects (329.1±21.8 versus 9.2±1.5; P<0.001),>1 gestational date sample of urine was available, and retrospective analysis showed a significant time-related increase of the urinary level of inositol phosphoglycan P-type 7 weeks before clinical diagnosis of preeclampsia. Urinary level of inositol phosphoglycan P-type increased after diagnosis indicating a possible pathophysiological threshold level and steeply decreased after delivery.
Key Words: preeclampsia • screening test • inositol phosphoglycan • biological marker • insulin resistance
Hypertension. 2007;49:84.
© 2007 American Heart Association, Inc.
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